its essential role in vir tually all cells and tissues examined

The unresponsiveness was dependent on TCR activation BAY 11-7082 because TT and TTchA6 cell lines produced comparable levels of IFN in response to activation by 12 0 tetradecanoylphorbol 13 acetate and ionomycin, To determine whether anergic TTchA6 cell lines con tain T reg 1 cells, TT specific effector CD4 T cell lines generated as described previously were rechallenged with TT and autologous APC inside the presence of increasing num bers of anergic TTchA6 cells. Results shown in Fig. 5 B,demonstrate that anergic TTchA6 cells suppressed the IFN production of effector TT specific CD4 T cells in a dose dependent manner, TTchA6 cell lines and control TT cell lines contained equivalent percentages of CD4 T cells expressing CD25, however the percentages of CD4 T cells expressing CD69 was decreased, This decrease re sulted in lower percentages of CD25 CD69 T cells within an ergic TTchA6 cell lines as in contrast to TT control cell lines. CTLA 4, GITR, and FOXP3 mRNA expression was similar in the anergized and nonanergized TT cell lines, From these data it can be concluded that TT specific suppressor T cell lines generated in the presence of chA6 mAb did not incorporate larger percentage of CD4 CD25 T reg cells. The entire Lymphatic system number of cytokines created by anergized TTchA6 cell lines was minimal. A neutralizing anti IL 10R mAb was added to the cultures, demonstrating that reductions by cells is medi ated by IL 10. Together, these data indicate that repetitive stimulation of CD4 T cells with TT within the presence of chA6 mAb results in the induction of T reg cells that are phe notypically and functionally equal to T reg 1 cells. OC000459 851723-84-7 ChA6 mAb modulates antigen specific CD8 T cell responses To check whether chA6 mAb may also modulate antigen spe cific CD8 T cells, PBMCs from HLA A 0201 individuals were stimulated in a mixed lymphocyte peptide,spe cific reaction with an immunodominant influenza A mother,trix proteins derived peptide within the presence or inside the absence of chA6 mAb. After two rounds of arousal, MLP countries were rechallenged with MP. Sixty 88 within the absence of chA6 mAb. MLPchA6 cells were less responsive to antigen stimulation than were their MLP counterparts, as shown by the decreased produc tion of IFN in response to MP. 58 66, Moreover, CD8 T cells produced in the presence of chA6 mAb dis,performed lower antigen specific cytotoxic activity than did con-trol CD8 T cells, MP.